Part:BBa_K4960033

pPayload promoter->Pdp1_NDT-3*GGSGG-Cre-GSSG-HiBiT->PAU_RS16570->PAU_RS16565->PAU_RS16560->PAU_RS240

a novel payload loading Cre to verify recombinant PVCs can be reprogrammed to both load and deliver non-native proteins into target cells

Usage and Biology

For the delivery mediated by PVCs,Pdp1 NTD(the N-terminal domain (NTD) of Pdp1)(BBa K4960016) plays a significant role in loading diverse payloads(including the wild type and novel type) into the PVC complex as a ‘packaging domain’.Therefore,it is rebuilt by the NTD fusion with Cre(BBa K4960023) to verify recombinant PVCs can be reprogrammed to both load and deliver non-native proteins into target cells . PAU_RS24015、PAU_RS16560、PAU_RS16565、 PAU_RS16570(BBa K4960017 BBa K4960018BBa K4960019BBa K4960020) may be involved in gene regulation and possibly contribute to the formation of PVCs in E.coil.
Generally,this part is responsible for producing Cre Payload and loading it into PVC complex in subsequent experiment to confirm previous studies that recombinant PVCs can be reprogrammed to both load and deliver non-native proteins into target cells.

Special Design

Fuse the NTD of Pdp1 with Cre(a novel protein for Payloads)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 910
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 596
  
• 1000
  COMPATIBLE WITH RFC[1000]