Part:BBa_K649000

LasR and 3OC12HSL regulated promoter.

In the presence of 3OC12-HSL lasR can bind to the operator of this promoter and activate the transcription of downstream gene, whereas in the absence of 3OC12-HSL lasR can't bind to the operator and can't activate the transcription.

To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. To know detailed infomation, please click here.

Effect of 3OC12-HSL induction on fluorescence intensity
This work is done by Takuya Tsubaki.

GFP expression after induction of 3OC12-HSL is 170 times as high as before.

We improved previous las promoters.

1. our assay of BBa_J64010
2. BBa_K091117
3. BBa_R0079

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Contribution of SCAU-China 2023

What have we done?

We improved this part (BBa_K649000[1]) by optimizing the codons for Escherichia coli expression. Codon optimization was done with the help of GUANGZHOU IGE BIOTECHNOLOGY LTD. We successfully characterized its performance in E. coli TOP10. For more detailed characterization results, please refer to our composite_part-Part:BBa_K4632024 [2]and Part: BBa_K649000[3].