DNA

Part:BBa_K4329007

Designed by: Yuxin He   Group: iGEM22_BS_United_China   (2022-09-30)
Revision as of 05:10, 7 October 2023 by Gracey (Talk | contribs) (→‎Usage and Biology)


sgRNA for targeting ATP synthase in E.coli

What is it?

CRISPR/Cas9 is a gene editing technology used to inhibit the expression from the targeted sequence inducing DNA to activate its self-repair process. sgRNA will be attached to the CRISPR/Cas9 to target the desired area.

Usage and Biology

This system (QS suicide system) is a system that will self-start when our engineering bacteria are lost to the nature.In this case, as we add this QS suicide system in to engineering bacteria, it can take precaution against the danger of the engineering bacteria’s leakage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None