Device

Part:BBa_K4593022:Design

Designed by: Jianfei Song   Group: iGEM23_BNDS-China   (2023-10-09)
Revision as of 22:02, 9 October 2023 by Registry (Talk | contribs)

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Integrative device of endolysin production-release module


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2672
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 3567
    Illegal XhoI site found at 4510
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5009
    Illegal AgeI site found at 5147
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ez


Source

The genes encoding SPN1S endolysin and Rz-like spanin (SPN1S_0028 and SPN1S_0029) originate from Salmonella Typhimurium phage SPN1S (GenBank accession number NC_016761)(Lim et al., 2012). The gene encoding LysDZ25 originates from S. aureus phage DZ25 (Chang et al., 2023).

References