Device

Part:BBa_K4593019:Design

Designed by: Jianfei Song   Group: iGEM23_BNDS-China   (2023-10-08)
Revision as of 23:36, 8 October 2023 by Jianfei Song (Talk | contribs) (→‎Source)


Endolysin combinative expression circuit in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2195
    Illegal NgoMIV site found at 3021
    Illegal NgoMIV site found at 3695
    Illegal AgeI site found at 2064
    Illegal AgeI site found at 3165
    Illegal AgeI site found at 3393
    Illegal AgeI site found at 3564
    Illegal AgeI site found at 3677
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed as a subpart of the "S. aureus in vivo elimination apparatus for E. coli" (BBa_K4593020). It could not show observable function independently due to the lack of a release system. The promoter, RBS, and codon are adjusted to be suitable for expression in E. coli.

Source

LysDZ25 is from bacteriophage DZ25[1], LysGH15 is from Bacteriophage GH15[2], and ClyC is a hybrid endolysin with CBD of LysPALS1 and EAD of LysSA12[3].

References