Device

Part:BBa_K4593019

Designed by: Jianfei Song   Group: iGEM23_BNDS-China   (2023-10-08)
Revision as of 21:04, 8 October 2023 by Jianfei Song (Talk | contribs)


Endolysin combinative expression circuit in E.coli

This part could constitutively express the 3 types of S. aureus targeting endolysin-LysDZ25, ClyC, and Lys GH15-to eliminate S. aureus in vitro. Each type of endolysin has different optimal working pHs and salt concentrations, making their efficiency complement each other, covering various lytic conditions in the human digestive tract.

Detailed characterization of each endolysin is documented in their respective basic parts.

Please see the design page for usage instructions.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2195
    Illegal NgoMIV site found at 3021
    Illegal NgoMIV site found at 3695
    Illegal AgeI site found at 2064
    Illegal AgeI site found at 3165
    Illegal AgeI site found at 3393
    Illegal AgeI site found at 3564
    Illegal AgeI site found at 3677
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None