Signalling

Part:BBa_K4634014:Design

Designed by: Ng Swee Hong   Group: iGEM23_Tsinghua   (2023-10-07)
Revision as of 05:22, 7 October 2023 by Registry (Talk | contribs)

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OmpA signal peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of OmpA is MKKTAIAIAVALAGFATVAQA.Using the GeneOptimizer online tool developed by ThermoFisher, we performed codon optimization and converted the protein sequence of OmpA into a DNA sequence (ATGAAAAAGACCGCAATTGCCATTGCAGTTGCACTGGCAGGTTTTGCAACCGTTGCACAGGCA) which is most suitable for expression in Escherichia coli. OmpA signal peptide should be fused at the N terminal of target protein.



Source

Movva NR, Nakamura K, Inouye M. Amino acid sequence of the signal peptide of ompA protein, a major outer membrane protein of Escherichia coli. J Biol Chem. 1980 Jan 10;255(1):27-9. PMID: 6985608.

References