Coding

Part:BBa_K4865000:Design

Designed by: Jing Shi   Group: iGEM23_OTIA-Hangzhou   (2023-08-22)
Revision as of 06:29, 22 August 2023 by Registry (Talk | contribs)

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EGF without start condon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 134


Design Notes

There is a polyclonal enzyme cleavage site at the C end of the EGF, where we insert other gene fragment.


Source

EGF from Homo sapiens and PelB from Bacillus sp. .

References