Coding

Part:BBa_K4085015

Designed by: yuanze Liu   Group: iGEM21_Ulink-SIP   (2021-10-21)
Revision as of 13:28, 21 October 2021 by Registry (Talk | contribs)

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WcaG

GDP-L-fucose is a key precursor for the synthesis of 2-FL, and it can be synthesized through two pathways. One is the de novo pathway naturally present in E. coli originating from colanic acid biosynthesis. Starting from fructose 6-phosphate in the central metabolism, it is converted into GDP-L-fucose via five enzymatic steps (ManA, ManB, ManC, Gmd, WcaG) .The gene can code for GDP-L-fucose synthase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 184
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None