RBS

Part:BBa_K4437500

Designed by: Marian Grell   Group: iGEM22_Calgary   (2022-10-12)
Revision as of 06:05, 12 October 2022 by Registry (Talk | contribs)

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DeadRBS (non-coding)

The DeadRBS is an RBS derived from a study by Kosuri et al. (2013), which determined how translational efficiency changes depending on the strength of the RBS used in E. coli (1). The DeadRBS was used as a negative control, or point of reference for the other RBS, as it has the least amount of protein translated (1). Although the DeadRBS (BBa_K4437500) has experimentally demonstrated to have a low affinity for the ribosome, it still is capable of producing trace amounts of protein, which is a tool that can be leveraged by future teams that want to produce trace amounts of certain genes in an operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None