Composite
Part:BBa_K4385017
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-06)
Zinc constitutive surface display
This part consists of the constitutive promoters J23100, OmpC (BBa_K4385003) and ZBP (BBa_K4385004), which is used to verify the function of the zinc ion surface display parts. The 6*His tag was added to this sequence to purify the recombinant protein.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 414
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1304
Illegal NheI site found at 1327
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 414
Illegal BglII site found at 122
Illegal BglII site found at 1439 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 414
Illegal SpeI site found at 573
Illegal PstI site found at 921
Illegal AgeI site found at 240 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |