Coding
Part:BBa_K4323000:Design
Designed by: Jessica Marinelli, Kevin Lim, Chun Hin Wong Group: iGEM22_UManitoba (2022-09-30)
Coding sequence for Aerolysin
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 206
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 75
Illegal AgeI site found at 313
Illegal AgeI site found at 1270 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We optimize codon for expression in Escherichia coli
Source
Aeromonas hydrophila