Part:BBa_K4146106
The complete expression box used for expressing caffeine.
The complete expression box used for expressing caffeine.
The gene of TcAncCS1 is derived from Cocobroma.Xanthine can be converted to 3-methylxanthine (3-mX) by TcAncCS1 from Theobroma. TcCS2 from C. sinensis has a higher propensity to recognize 3-mX and can convert 3-mX to theobromine. We found that there are many ways to synthesis caffeine. The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated and a sequence consisting of xanthine →3-methylxanthine → theobromine → caffeine. In three of the four reactions, the gene encoding N-methyltransferase was involved.We chose the second way. CS stands for caffeine synthase, while the TcAncCS1 ancestral gene is derived from Cocobroma, the duplication of TcAncCS1 at node O leads to TcAncCS2, but the kinetic parameters of TcAncCS2 and its progeny enzymes predict that experts will be favored because the 3 methyl Xanthine does not compete for active site binding. We introduced this plasmid gene to facilitate the synthesis of caffeine in engineered strains, thereby regulating the balance of scalp oil.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2479
Illegal PstI site found at 2602 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2479
Illegal PstI site found at 2602 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2479
Illegal PstI site found at 2602 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2479
Illegal PstI site found at 2602
Illegal NgoMIV site found at 643
Illegal NgoMIV site found at 1741 - 1000COMPATIBLE WITH RFC[1000]
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