Composite

Part:BBa_K209422:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-02)
Revision as of 21:36, 5 October 2009 by Katja (Talk | contribs) (→‎Design Notes)

SSFYFP-Rs1.3-Beta Pix


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 721
    Illegal XbaI site found at 5337
    Illegal XbaI site found at 6759
    Illegal PstI site found at 2984
    Illegal PstI site found at 5492
    Illegal PstI site found at 5758
    Illegal PstI site found at 6079
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 239
    Illegal NheI site found at 505
    Illegal PstI site found at 2984
    Illegal PstI site found at 5492
    Illegal PstI site found at 5758
    Illegal PstI site found at 6079
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2766
    Illegal BamHI site found at 706
    Illegal BamHI site found at 4590
    Illegal BamHI site found at 4804
    Illegal XhoI site found at 4117
    Illegal XhoI site found at 5641
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 721
    Illegal XbaI site found at 5337
    Illegal XbaI site found at 6759
    Illegal PstI site found at 2984
    Illegal PstI site found at 5492
    Illegal PstI site found at 5758
    Illegal PstI site found at 6079
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 721
    Illegal XbaI site found at 5337
    Illegal XbaI site found at 6759
    Illegal PstI site found at 2984
    Illegal PstI site found at 5492
    Illegal PstI site found at 5758
    Illegal PstI site found at 6079
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2722
    Illegal BsaI.rc site found at 5542
    Illegal BsaI.rc site found at 6844
    Illegal SapI.rc site found at 116
    Illegal SapI.rc site found at 5148


Design Notes

Cloned into pDONR221.

Source

References