Reporter
Part:BBa_K4242013
Designed by: zongheng Wei Group: iGEM22_CUG-China (2022-09-29)
Pci+RBS+fleQ+TT+ Pci+Ppel+RBS+gfp+TT(pHYD-3)
Uses factor Pci(BBa_R0051), strong RBS(BBa_J34801), protein FleQ coding region(BBa_), Pci(BBa_R0051) and Ppel(BBa_ ), TT(BBa_), RBS(BBa_), gfp coding region(BBa_E0040), and TT(BBa_). This part is a device to construct the expression of protein FleQ. FleQ is a transcription factor combined with c-di-GMP that can regulate genes about the expression of flagella biosynthesis and exopolysaccharide.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 943
Illegal XhoI site found at 1396 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 336
Illegal NgoMIV site found at 1344
Illegal NgoMIV site found at 1356
Illegal NgoMIV site found at 1432
Illegal NgoMIV site found at 1517
Illegal AgeI site found at 745 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2567
Illegal SapI.rc site found at 358
Illegal SapI.rc site found at 1291
Illegal SapI.rc site found at 1300
[edit]
Categories
Parameters
None |