Coding
CycA
Part:BBa_K4339000:Design
Designed by: Grace Poulton Group: iGEM22_Exeter (2022-07-06)
Non-polar amino acid transporter (CycA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 340
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was modified to remove forbidden restriction sites to allow for type IIS cloning.
Source
The CycA sequence originates in a K-12 strain of E. coli.