Composite
oxySp-GFP

Part:BBa_K4225002:Design

Designed by: Leon Ritchie Salim   Group: iGEM22_HKUST   (2022-10-07)
Revision as of 06:56, 7 October 2022 by Registry (Talk | contribs)

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GFP that is induced by oxySp, a H2O2 inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 811


Design Notes

Prefix and Suffix are added, and spacers are also added between the parts.


Source

ATCGAGAATTCGCGGCCGCTTCTAGAGTTCATTATCCATCCTCCATCGCCACGATAGTTCATGGCGATAGGTAGAATAGCAATGAACGATTATCCCTATCAAGCATTCTGACTGAGCATTGCTCACAACTTTATCTGAGAATAGTCAATCTTCGGAAATCCCAGGTGAAAGAGGAGAAATAATAAAAGGTCCCGATGCGCAAGGGTGAGGAGCTTTTCACCGGCGTCGTGCCTATCTTGGTCGAGCTGGACGGCGACGTTAATGGGCACAAGTTCTCAGTCAGCGGGGAAGGGGAAGGTGACGCGACATACGGGAAACTGACCCTTAAATTTATTTGTACGACAGGCAAATTACCAGTTCCTTGGCCCACACTGGTGACTACGTTTGGTTATGGTGTGCAGTGTTTTGCTCGCTATCCGGATCATATGAAGCAGCATGATTTTTTCAAGTCAGCTATGCCCGAAGGTTATGTACAAGAGCGTACTATCTTTTTCAAGGATGATGGAAATTATAAAACGCGTGCTGAGGTTAAATTTGAGGGGGATACACTGGTAAACCGCATTGAATTAAAAGGCATTGATTTCAAGGAAGACGGGAATATTCTGGGTCACAAGTTAGAGTACAACTATAATTCGCATAACGTCTATATCATGGCAGACAAACAGAAGAACGGAATTAAGGTAAACTTTAAGATCCGCCACAATATCGAGGACGGTAGTGTCCAACTTGCGGACCACTACCAGCAAAACACACCAATTGGCGATGGGCCTGTTTTATTGCCAGACAACCACTACCTTAGCACACAATCTGCGCTGTCGAAAGATCCAAACGAGAAGCGTGATCACATGGTCCTGCTTGAGTTCGTGACAGCAGCAGGTATCACCCACGGTATGGACGAGTTGTACAAGTGATAAAGATTACTACTGATACCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATATACTAGTAGCGGCCGCTGCAGATCGA

References