Translational_Unit

Part:BBa_K4239008:Design

Designed by: Guillaume FULCONIS   Group: iGEM22_INSA_Lyon1   (2022-10-05)
Revision as of 20:29, 5 October 2022 by Registry (Talk | contribs)

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Enhanced luciferase substrate forming subunits fiatluxCD and the promoter J23117


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 50
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1366


Design Notes

Every Igem restriction sites (EcoR1, Xba1, Spe1 and Pst1) were removed from the part. Codon optimization is done for E.coli and D.solani.



Source

fiatlux is made up of fiatluxABE, fiatluxCD and the promoter J23117.

References