Coding
Cyt2Ba

Part:BBa_K4321000

Designed by: Kulay Janneh   Group: iGEM22_Guelph   (2022-09-28)
Revision as of 17:27, 28 September 2022 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Cytotoxic Protein 2Ba (Cyt2Ba)

Since its discovery in 1901, Bacillus thuringiensis (Bt) has been identified as encoding over 800 insecticidal crystal proteins(ICP). These ICPs include members of the crystal (Cry) and cytolytic (Cyt) family.

Cyt proteins can be split into three subfamilies (Cyt1, Cyt2, and Cyt3) and all have the same mode of action. They are produced as protoxins and ultimately activated by species-specific proteolytic cleavage in the midgut of insect targets. Activated Cyt2Ba binds to membrane receptors to signal increased cell permeability. This promotes pore formation in the lining of the midgut and ultimately kills the target insect.

Unlike Cry proteins, Cyt proteins are toxic only to Diptera species and can potentially suppress the resistance to Cry proteins. These proteins can be used in their native structure or be modified to target insect species outside of the Diptera suborder.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds
//cds/ligand
//cds/membrane/lysis
//function/celldeath
Parameters
protein