Part:BBa_K4140013

Sensing Device

The Vesicular Stomatitis Virus G (VSV G) protein is a typical type III viral fusion protein that, when present in cell-free environments, forms complexes with plasmid DNA and particles that resemble MLV retroviruses and improves DNA transfection.

Characterization of Mutational Landscape

After creating a multiple sequence alignment of the protein sequence and predicting mutational landscapes, the effect of these mutations on the evolutionary fitness of the protein is tested. The prediction of the mutational landscape by saturation mutagenesis of the VSVg-Fusogen protein. The (Y148H) mutation, as depicted in the chart, had the greatest score when compared to other mutations. On the other hand, it's clear that the (A56T) had the least evolutionary fitness for VSVg-Fusogen protein. As displayed in Figure(1)

Figure 1. (shows the mutational landscape of the VSVg-Fusogen protein.)

References

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 771
  Illegal BglII site found at 1113
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 577
  
• 1000
  COMPATIBLE WITH RFC[1000]