Part:BBa_K4235025

Ampicillin resistance gene cassette

Introduction Ampicillin is one of the most common selection markers used in synthetic biology. Its structure contains a β-lactam ring that inhibits proteins involved in bacterial peptidoglycan synthesis. As a result, bacteria cannot synthesize new peptidoglycan which disrupts the formation of cell walls and leads to cell death. The ampicillin resistance gene encodes for the enzyme β-lactamase, which cleaves the β-lactam ring and inactivates the ampicillin, thereby providing resistance to the bacteria.

Usage and Biology

This part is the entire circuit of the Ampicillin Resistance gene which is used within the plasmid (pFastBac-YmBac2) and it confers resistance to Ampicillin. The expression of the ampicillin coding sequence is driven by the AmpR promoter (add link). This marker was used for the selection of successful transformants after cloning our gene insert (add link) in the plasmid pFastBac.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 821