Composite

Part:BBa_J428100

Designed by: 2021_Engineering_Committee   Group: 2021 Engineering Committee   (2022-06-06)
Revision as of 20:44, 8 June 2022 by Alejovigno (Talk | contribs)


Interlab_NegC Non-fluorescent negative control for InterLab studies 2022. It uses the same elements as the fluorescent constructs but expresses a non-fluorescent protein (LuxR). It includes a region upstream of the promoter to insulate transcription, a promoter (J23106), a RiboJ to insulate translation, the B0032 RBS, the CDS for the LuxR protein, and an efficient transcriptional terminator (rnpb_T1). It imposes the same burden on the bacteria for a more fair growth comparison between the different devices.


Interlab_2022_DoubleConstructs and Interlab_2022_ThreeColors

J428100.
Figure 1. SBOL Visual representation of the device J428100 from 2022 Interlab studies.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 71
    Illegal NheI site found at 94
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None