Part:BBa_K116401

external phosphate sensing promoter

Promoter of phoB in E.coli, an external phosphate regulated promoter that can be activated when encountering phosphate limitation.

Experience (Lambert_GA 2021)

Utilizing the modified phosphate range and characterization protocol (See: Modified Protocol), we characterized the BBa_K2447000 phosphate sensor. The characterization curve (Figure 1) showed a linear, negative trend throughout phosphate concentrations ranging from 0-100μM. This data closely paralleled the predictive ODE model created by our 2020 team and the previous characterization data by NUS Singapore iGEM 2017.

Figure 1. Characterization curve for BBa_K2447000 for phosphate concentrations between 0μM and 100μM.

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]