Part:BBa_K4023001

Optimized glpF

glpF codes for a 28kDa¹ transmembrane glycerol uptake facilitator protein found in E.coli strain. glpF protein catalyze transmembrane diffusion of glycerol and some linear polyhydric alcohols in E.coli¹. glpF protein also allows Arsenite to be transported into E.coli. This is as Arsenite is protonated at physiological pH and forms As(OH)3 as a major species. As(OH)3 is chemically analogous to glycerol².

The glpF sequence was obtained from iGEM09_Groningen. We optimized the sequence using IDT optimization tool and Benchling for expression within an E.coli host.

References

1. Fu, D., Libson, A., & Stroud, R. (2002, April). The structure of G1pF, a glycerol conducting channel. In Ion Channels: From Atomic Resolution Physiology to Functional Genomics: Novartis Foundation Symposium 245 (Vol. 245, pp. 51-65). Chichester, UK: John Wiley & Sons, Ltd.

2. Bhattacharjee, H., Mukhopadhyay, R., Thiyagarajan, S., & Rosen, B. P. (2008). Aquaglyceroporins: ancient channels for metalloids. Journal of biology, 7(9), 1-6.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 183