Reporter

Part:BBa_K3735666

Designed by: Guolong Shi   Group: iGEM21_NAU-CHINA   (2021-10-21)
Revision as of 16:27, 21 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


enhanced BBa_J04450

After learning about the Dual UTR, we want to utilize it to improve an existing measurement part so that subsequent researchers can detect fluorescence more efficiently and accurately. Then we chose the BBa_J04450 which expresses mRFP under the induction of IPTG. We intend to add the Dual UTR between mRFP(BBa_E1010) and lac promoter(BBa_R0010) to improve its expression. Thereupon, we construct the following part(BBa_K3735666).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 882
    Illegal AgeI site found at 994
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None