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Part:BBa_K2364042

Designed by: Asterios Arampatzis   Group: iGEM17_Greece   (2017-10-26)
Revision as of 18:52, 20 October 2021 by Shinichi (Talk | contribs)

Τ21

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Supplementary Experiment

Group: CSU_CHINA 2021

Author: Xingjun Zhao

T21 is called miR21T in our project, it is a DNA sequence that can be the target of miRNA21. We have enhanced the method used by iGEM17_Greece in 2017 and inserted a piece of T21 in target gene 5’UTR and 4 pieces of T21s in 3’UTR, significantly improved the efficiency of miR21 targeting T21 and suppressing the expression of target gene, meet our feedback suppression requirement in our loop.

Fig.1 Electrophoretic diagram of miR21T-LUC-4XmiR21T PCR product

Fig.2 miR21 inhabited the expression of reporter LUC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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