Coding

Part:BBa_K4023000

Designed by: Jasmine Wee   Group: iGEM21_Washington   (2021-09-30)
Revision as of 04:05, 1 October 2021 by Registry (Talk | contribs)

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Modified MTIA

Metallothionein are small proteins containing a high percentage of conserved cysteine residues that bind a variety of different heavy metals. Metallothionein-IA is a metallothionein protein found in humans. It is broadly expressed in the liver, which matches its known function of detoxification of heavy metals. It is also known to protect against oxidative stress and carcinogens.

The 7 metal binding sites, (3 Zinc and 4 Cadmium) in Human Metallothionein IA have been modified to be more specific to Arsenic. Hence, it can be expected to sequester As III with higher efficacy as compared to wildtype metallothionein-IA.

The MTIA gene has been optimized to express metallothionein IA proteins in E.coli for remediation of As III from the environment.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None