Part:BBa_K3995003

P7_AtzR

P7_AtzR

Usage and Biology

P7_AtzR is a coding sequence for expressing atzR. Cyanuric acid combination with AtzR can activate amilGFP expression to show the signs of fluorescence. The content of cyanuric acid is detected by detecting the fluorescence concentration. So P7_AtzR should be used with Pprovoin5_amilGFP.

Figure1. Figure 1 Genetic Design of our Sensor.

Construct design

The atzR is under T7 promoter. The P7_atzR is inserted in the pUC57 mini vector (Figure 3).

Figure 2. P7_AtzR box..

Figure 3. Schematic maps of P7_AtzR (sensor plasmid).

BBa_K3995000

Name: P7 Base Pairs: 19bp Origin: T7 phage Properties: A promoter for initiation of the transcription.

Usage and Biology

The promoter is recognized for binding and initiation of the transcription. Placed before a gene, it promotes its transcription.

BBa_K3995001

Name: AtzR Base Pairs: 969bp Origin: Pseudomonas sp. Properties: A protein binding cyanuric acid

Usage and Biology

BBa_K3995001 is a coding sequence of atzR. Cyanuric acid combining with atzR can activates Pprovoin5 promoter. So atzR can act as a sensor.

BBa_K4030002

Name: TT Base Pairs: 140bp Origin: Escherichia coli Properties: Transcription terminator

Usage and Biology

It is an transcription terminator derived from the E.coli rrB rRNA operon

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 412
  Illegal EcoRI site found at 955
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 412
  Illegal EcoRI site found at 955
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 412
  Illegal EcoRI site found at 955
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 412
  Illegal EcoRI site found at 955
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 412
  Illegal EcoRI site found at 955
  Illegal AgeI site found at 802
  
• 1000
  COMPATIBLE WITH RFC[1000]