Part:BBa_K337028
Tet Repressor
The TetR binds to the Tet Operator and by that causes knockdown auf gene expression. (BB-2, RFC12).
Improvement by iGEM ZJUT-China 2021
Figure 1. Schematic of hydrolysis by ClpXP protein.
As the improvement of the 10_Heidelberg registered part (BBa_K337028: tet Repressor) , the degradation tag ssrA is added to the 3' end of the tetR (Figure 1) , hence the newly registered partBBa_K3885126.
Results
Figure 2. Schematic of gene circuits and kinetics of tetR inhibition in the Cell-Free system.
(A) The gene circuits contains three parts, sigma 28 activates promoter P28 to express tet repressor with ssrA tag, which inhibits the expression of deGFP.
(B) The gene circuits contains an additional part P70-ClpXP that makes the tetR repressor degrade, and degfp expresses.
(C) Group 1 carried tetR without ssrA and ClpXP; Group 2 carried tetR with ssrA; Group 3 didn't carry tetR as positive control; Group 4 carried tetR with ssrA and ClpXP in comparison with Group 1.
Therefore, we gave the tetR a new function and improved this part.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 10
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 652
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 10
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 10
- 1000COMPATIBLE WITH RFC[1000]
None |