Template:BioE140LSpr09-Autoaggregation

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Autoaggregation: Cell-Cell Adhesion Assay


The purpose of this assay was to determine the ability of recombinant E.coli to aggregate when expressing IILK (leucine zipper) peptides on their outer membranes. The IILK peptide was displayed on the outer membrane via fusion to different displayer peptides. This assay tested the efficacy of the carrier proteins as fusions to functional IILK leucine zippers.
The following composite parts were tested during this assay: 

M10218       {Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<CPG_L6!}{dblTerm}
M10219       {Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<eCPX!}{dblTerm}
M10220       {Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<upaG_short!}{dblTerm}
M10221       {Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<Ag43_short!}{dblTerm}
M10222       {Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<espP(beta)!}{dblTerm}
M10223       {Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<ehaB!]{dblTerm}
M10224       {Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<CPompX!}{dblTerm}
M10225       {Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<TshA!}{dblTerm}

Procedure