Regulatory

Part:BBa_K3457003

Designed by: Yixian Yang   Group: iGEM20_QHFZ-China   (2020-10-03)
Revision as of 01:17, 20 October 2021 by Enshixu (Talk | contribs)


T7 promoter

T7 promoter of pet-modification vector

Contribution

Group: QHFZ-China iGEM 2020

This part is a basic part. QHFZ-China 2020 measured it in their composite parts, K3457032, K3457033, K3457034, K3457035, K3457036, K3457037, K3457039, K3457042, K3457043.

Contribution from iBowu-China 2021

In iGEM 2021, Team iBowu-China adopted T7 promoter to use in combination with LacO and gusA sequence from E. coli and other sources to express an enzyme β-glucuronidase. In our design, we tested the use of this "short" T7 promoter and noted the difference with a complete T7 promoter [1].

Figure.1. The plasmid constructions used for the expression of this part. This part encodes an enzyme β-glucuronidase.


References

[1] Jones, J., Vernacchio, V., Lachance, D. et al. ePathOptimize: A Combinatorial Approach for Transcriptional Balancing of Metabolic Pathways. Sci Rep 5, 11301 (2015). https://doi.org/10.1038/srep11301




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None