Coding

Part:BBa_K3815007

Designed by: Hiroto Koga   Group: iGEM21_Kyoto   (2021-10-16)
Revision as of 09:03, 17 October 2021 by Shiotsu (Talk | contribs)


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This part is used His tag to purify the peptides. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein.In this part, the purpose protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.

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