Part:BBa_K3930008

Gene coding for the Linalool synthase Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 1135
  Illegal PstI site found at 23
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal SpeI site found at 1135
  Illegal PstI site found at 23
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 1135
  Illegal PstI site found at 23
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 1135
  Illegal PstI site found at 23
  
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

This sequence codes for the linalool synthase, which transforms GPP into the flower-smelling compounds linalool, smelling like flower. The sequence derived from the Lavandula angustifolia genome. We take advantage of the publication of (Amiri et al., 2016).

There is already (BBa_K3052002) on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but our is codon optimized for translation in S. cerevisiae.

Testing

This part is one of the component of the pFLEUR part (BBa_K3930000) and has been succesfully cloned into the LycoYeast S. cerevisiae strain genome. The Toulouse team could not test this part for reasons of time, and preferred to focus on the part characterizations that allow the production of α and β-ionone for the floral aspect of the violet perfume.

References

1. Amiri P, Shahpiri A, Asadollahi MA, Momenbeik F, Partow S. 2016. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett. 38(3):503–508. doi:10.1007/s10529-015-2000-4.