Coding

Part:BBa_K3989010

Designed by: Tianyu Xu   Group: iGEM21_UZurich   (2021-09-30)
Revision as of 16:29, 16 October 2021 by ZhangSongyuan (Talk | contribs)


plant immune elicitor flg22 fused with ClyA for OMV display

In this construct, outer membrane-associated protein ClyA is used to display the plant immune elicitor flg22 to trigger plant immune responses. To make flg22 better recognized by plant immune receptor FLS2, we put mutiple linkers and a 3×FLAG tag between ClyA and flg22 to minimize the steric hindrance.

By doing western blot using anti-FLAG antibody, we proved that ClyA-elicitor fusion proteins indeed exist in outer membrane vesicle (Figure 1).

ClyA in omp8 OMV.jpeg

Figure 1. ClyA protein with or without different PAMPs exists in outer membrane vesicles of E. coli omp8 strain.

Omp8 OMVs on col-0.jpeg

Figure 2.

TolB OMVs on col-0.jpeg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1105
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1105
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1105
    Illegal NgoMIV site found at 1099
    Illegal AgeI site found at 1063
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None