Generator

Part:BBa_K3962343

Designed by: Siheng Li   Group: iGEM21_Leiden   (2021-10-11)
Revision as of 15:51, 11 October 2021 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


arabinose-mediated inducible expression of toxin RelE

This construct is composed of L-arabinose-mediate inducible promoter pBAD and toxin RelE. RelE/RelB is a type II toxin/antitoxin system originated from E. coli. In our project, we used this construct to test the toxicity of toxin RelE, which provided us with more insights to regulate the toxin-antitoxin system for preventing horizontal gene transfer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None