Coding

Part:BBa_K3930024

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 12:42, 8 October 2021 by Registry (Talk | contribs)

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LCYe-ofCCD1m fusion with a LGS linker to produce α-ionone in Saccharomyces cerevisiae

This sequence codes for an enzymayivc fusion between LcyE that transforms Lycopene into epsilon-carotene, and ofCCD1 that transforms epsilon-carotene into alpha-ionone. Those two sequences are codon optimized for an expression into S.cerevisiae. The LcyE sequence comes from Latuca sativa and ofCCD1 comes from Osmanthus fragrans.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 282
    Illegal BglII site found at 812
    Illegal BamHI site found at 396
    Illegal BamHI site found at 2535
    Illegal BamHI site found at 3387
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None