Regulatory

Part:BBa_K3887008

Designed by: Ran Wang   Group: iGEM21_BJU_China   (2021-10-01)
Revision as of 08:05, 13 October 2021 by Ruijuan Xiang (Talk | contribs)


LacO-scar-T7-LacO Promoter

This is an optimized part of BBa_K2406020 .

Decriptioon

T7 promoter is commonly used in iGEM projects, [Part:BBa_K2406020|BBa_K2406020]] designed by iGEM17_Edinburgh_UG was an inducile T7-LacO promoter for gene expression. In our project, we also used T7-LacO promoter to control the expression of halogenase. However, we found high basic leakage of this promoter, which affected the results of our experiments, so we improved this promoter in our project. It is recognized that an excellent promoter should possess exceptional switch ratio: it should have lower background leakage in the "off" state and have obvious expression in the "on" state. In order to reduce the leakage and to ensure the repressor can bind better to the LacO site, we added another LacO site in the T7-LacO promoter as the figure below shows:

T--BJU China--Fig.1 mprovement.png

Fig.1 Improved promoter and original promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None