Coding

Part:BBa_K3930020

Designed by: Thomas Gaudin   Group: iGEM21_Toulouse_INSA-UPS   (2021-10-08)
Revision as of 08:18, 8 October 2021 by Registry (Talk | contribs)

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G418 selective cassette

This cassette allows the selection of Yeast transformant upon addition of G418 into the media of culture. The sequence of the gene carrying the resistance is codon optimized for an expression into S.cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 873
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 722
    Illegal SapI.rc site found at 932


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Categories
Parameters
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