Protein_Domain

Part:BBa_K3732000

Designed by: Jiehui Ye   Group: iGEM21_FAFU-CHINA   (2021-09-20)
Revision as of 20:02, 20 September 2021 by Registry (Talk | contribs)

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photosensitive degron (psd) module.

The psd module can be attached to the carboxy terminus of target proteins that are localized to the cytosol or nucleus to obtain light control over their stability. Blue light induces structural changes in the LOV2 domain, which in turn lead to activation of the degron and thus proteasomal degradation of the whole fusion protein. Variants of the psd module with diverse characteristics are useful to fi ne-tune the stability of a selected target at permissive (darkness) and restrictive conditions (blue light).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None