Coding

Part:BBa_K3852004

Designed by: Yuan Cao   Group: iGEM21_BIT-China   (2021-10-01)
Revision as of 05:56, 1 October 2021 by Registry (Talk | contribs)

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T2R4

TAS2R46 belongs to the large TAS2R receptor family. TAS2Rs are expressed on the surface of taste receptor cells and mediate the perception of bitterness through a G protein-coupled second messenger pathway. After being stimulated by bitter substances, the bitter receptors activate -gustducin and effector enzyme PDE, and reduce the concentration of intracellular second messenger called ring nucleoside acid(cNMP). After that, calcium channels in the cell membrane were opened to make extracellular calcium ions flow in, and the concentration of intracellular calcium ions increased. Finally, the membrane depolarizes, releasing neurotransmitters.In our experiments, we used this gene to express bitter taste receptors in Saccharomyces cerevisiae


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None