Part:BBa_K3852002

Pfba1+T2R1+eGFP

 This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. This intronless taste receptor gene encodes a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is mapped to chromosome 5p15, the location of a genetic locus (PROP) that controls the detection of the bitter compound 6-n-propyl-2-thiouracil.

 Bitter taste signaling in humans is mediated by a group of 25 bitter receptors (T2Rs) that belong to the G-protein coupled receptor (GPCR) family. However, the molecular targets or receptors in humans for these bitter peptides were poorly characterized and least understood. The molecular models showed that the bitter peptides bind within the same binding pocket on the receptor. The ligand binding pocket in T2R1 is present on the extracellular surface of the receptor, and is formed by the transmembrane helices 1, 2, 3 and 7 and with extracellular loops 1 and 2 forming a cap like structure on the binding pocket. Experiments show that bitter taste receptor T2R1 can be activated by dipeptides and tripeptides.In our experiments, we used this gene to express bitter taste receptors in Saccharomyces cerevisiae.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 638
  Illegal XbaI site found at 1345
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 638
  Illegal XbaI site found at 1345
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 638
  Illegal XbaI site found at 1345
  
• 1000
  COMPATIBLE WITH RFC[1000]