Tag
6xHis-tag

Part:BBa_K3934015

Designed by: iGEM Uppsala 2021   Group: iGEM21_Uppsala   (2021-09-30)
Revision as of 12:21, 30 September 2021 by EmelieUU21 (Talk | contribs)


6xHis-Tag for protein purification

A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None