Part:BBa_K3832008

Induced expression circuit of aroG (Mutant s211f)

This Part is the Induced expression circuit of CDS aroG (Mutant s211f) [1].It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 76
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2469
  Illegal NheI site found at 2492
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 1034
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 76
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 76
  
• 1000
  COMPATIBLE WITH RFC[1000]