Composite

Part:BBa_K3832001:Design

Designed by: Hongyu Chen   Group: iGEM21_XJTU-China   (2021-09-26)
Revision as of 06:57, 26 September 2021 by SuperCRISPR (Talk | contribs) (→‎Design Notes)


Induced expression circuit of aroG (Mutant s211f)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2554
    Illegal NheI site found at 2577
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2541
    Illegal XhoI site found at 1146
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 453


Design Notes

This part can be directly linked to the vector plasmid. No extra Promoter and Repressor gene(lacI) are required.

The following subparts are included in order：

lacUV5 promoter， lac operator， BBa_B0034(RBS), aroG-s211f(CDS), T7 terminator T7Te terminator, rrnB T1 terminator, lacI(CDS), eSD, BBaJ23100(promoter)

Source

The sequence of the parts used in the device are gained from NCBI database, except the sequence of BBa_B0034 is gained from iGEM parts collections. Partial of the subparts are de-novo synthesized. All the subparts are assembled into this device by using GoldenGate assembly.

References