Coding
Part:BBa_K3972000
Designed by: Ingrid Kolen, Daniek Hoorn, Werner Doensen Group: iGEM21_TU-Eindhoven (2021-09-20)
TtrS
E. coli-codon-optimized TtrS(BBa_K3972000) and TtrR (BBa_K3972001) are two basic parts which are derived from the two-component system of the marine bacterium Shewanella baltica. TtrS is the membrane-bound sensor kinase (SK) which can sense tetrathionate outside the cell, and TtrR is the DNA-binding response regulator (RR)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1561
Illegal PstI site found at 1013
Illegal PstI site found at 1028
Illegal PstI site found at 1084
Illegal PstI site found at 1381
Illegal PstI site found at 1408 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1561
Illegal NheI site found at 1309
Illegal PstI site found at 1013
Illegal PstI site found at 1028
Illegal PstI site found at 1084
Illegal PstI site found at 1381
Illegal PstI site found at 1408 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1561
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1561
Illegal PstI site found at 1013
Illegal PstI site found at 1028
Illegal PstI site found at 1084
Illegal PstI site found at 1381
Illegal PstI site found at 1408 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1561
Illegal PstI site found at 1013
Illegal PstI site found at 1028
Illegal PstI site found at 1084
Illegal PstI site found at 1381
Illegal PstI site found at 1408
Illegal AgeI site found at 783 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds
//chassis/prokaryote/ecoli
//chassis/prokaryote/ecoli
biology | S. Baltica |
protein | TtrS |