Device

Part:BBa_K112808

Designed by: Jin Huh   Group: iGEM08_UC_Berkeley   (2008-10-31)
Revision as of 06:08, 31 October 2008 by Registry (Talk | contribs)

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Enterobacteria phage T4 Lysis Device - no promoter

This is the lysis device using three genes from enterobacteria phage T4: lysozyme, holin, and antiholin. Various promoters can be installed with this device to control the lysis of bacteria. Antiholin has its own constitutive promoter to prevent the formation of holin multimers from basal expression. When the device is off, higher expression of antiholin compared to holin is required for better stability of the device.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1350
    Illegal NheI site found at 1373
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 955
    Illegal AgeI site found at 1025
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1606


[edit]
Categories
//function/celldeath
Parameters
None