Plasmid

Part:BBa_K112245

Designed by: Molly Allen   Group: iGEM08_UC_Berkeley   (2008-10-28)
Revision as of 21:46, 28 October 2008 by Registry (Talk | contribs)

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{pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid

This part was inserted into the K112995 plasmid between ccdB and the attR2 site.

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3242
    Illegal PstI site found at 881
    Illegal PstI site found at 5225
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4084
    Illegal PstI site found at 881
    Illegal PstI site found at 5225
    Illegal NotI site found at 141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 212
    Illegal XhoI site found at 5265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3242
    Illegal PstI site found at 881
    Illegal PstI site found at 5225
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3242
    Illegal PstI site found at 881
    Illegal PstI site found at 5225
    Illegal AgeI site found at 3674
    Illegal AgeI site found at 3998
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 762
    Illegal BsaI.rc site found at 4366


[edit]
Categories
//function/recombination
Parameters
None