Coding

Part:BBa_K4033004

Designed by: Chenxi Wang   Group: iGEM21_BHSF   (2021-08-24)
Revision as of 03:22, 25 August 2021 by Registry (Talk | contribs)

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DnrF for coding

The DnrF gene, responsible for conversion of aklavinone to ɛ-rhodomycinone via C-11 hydroxylation, was mapped in the daunorubicin (Dnr) gene cluster of Streptomyces peucetius ATCC 29050. The DnrF gene was sequenced and should encode a protein of 489 amino acids with a molecular mass of 52 kDa. The heterologous expression of DnrF in Escherichia coli demonstrated that the gene encodes a NADPH-dependent hydroxylase catalysing the hydroxylation of aklavinone to yield ɛ-rhodomycinone. It is used as monooxygenase in our design which can finally let dcll become carminic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 508
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 508
    Illegal NotI site found at 1219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1074
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 508
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 508
    Illegal AgeI site found at 722
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None