Part:BBa_K4033001
ZhuI
ZhuI is a monodomain ARO/CYC that promotes the regiospecific C7–C12 first-ring cyclization for unreduced polyketide chains of variable lengths (C16 and C18–C20). And C7–C12 specificity for first-ring cyclization is unique among nonreducing type II PKS systems.
Biology and Usage
ZhuI encodes the cyclases that catalyzes formation of the C7-C12 first ring closure, which is unique among nonreducing type II PKS systems. ZhuI is proposed to catalyze aldol condensation between C7 and C12 (followed by aromatization) to yield intermediate SEK4b, which is then transferred to ZhuJ for second-ring cyclization between C5 and C14 (followed by aromatization) to give intermediate SEK4. After third-ring cyclization between C2 and C15, presumed to occur spontaneously, flavokermesic acid anthrone forms
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