Part:BBa_K1075032
ccdA - Antitoxin in ccdA/ccdB system
CcdA inhibits the toxin ccdB by binding to it and thus represses cell death . There is no stop codon because we used it in fusion with the E.Coli ssrA(DAS+4) tag.
Usage and Biology
The ccd module is a toxin-antitoxin (TA) system. The module naturally occurs on the F Plasmid in Escherichia coli bacteria and is essential for their survival. Normally the toxin ccdB is inactivated by the presence of the antitoxin ccdA in the form of a ccdAB complex. If ccdA is no longer available, ccdB inhibits DNA gyrase which leads to cell death. Gyrase is a type IIA topoisomerase specific to E. coli and is able to produce negative DNA supercoiling by making a double-strand break in the DNA and religating it. CcdB stabilizes the gyrase cleavage complex and thus blocks the catalytic function of the gyrase. That means that the gyrase remains bound to the DNA and the cleaved DNA is not religated. DNA- and RNA polymerases can’t copy the DNA anymore and cell proliferation as well as protein biosynthesis is stopped. The double-stranded breaks in the DNA initiate cell death. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635281/][http://www.ncbi.nlm.nih.gov/pubmed/?term=A+Common+Origin+for+the+Bacterial+Toxin-Antitoxin+Systems+parD+and+ccd]
sequence and features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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